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Human Sample ID Kit
The Human Sample ID Kit is an easy-to-use sequencing-based genotyping assay that targets polymorphic SNPs and gender markers, creating a highly specific intrinsic genetic label for each DNA sample. It comes in 2 versions, with either 49 or 13 markers. In a single reaction, short read sequencing-ready libraries are generated compatible. The Human Sample ID Kit is designed to be compatible with popular exome enrichment platforms and has been validated for both high-quality genomic, low-quality formalin-fixed paraffin-embedded (FFPE) and cell-free DNA templates.
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Advantages
Excellent coverage uniformity
Easy integration in WES and WGS
Single reaction workflow
Analysis software with report
Key features
Extremely low random probability match of 1 in 27 quadrillion individuals
Ambient temperature shipment
Maximum compatibility with exome enrichment kits
Convenient single-use Unique Dual Index plates
Products
Highlights for Human Sample ID Kit
Sample type
blood DNA, FFPE tissue DNA, plasma cfDNA
Input requirements
2 - 20 ng
Total time
3 hours
Hands-on time
20 minutes
Number of markers
49 (incl. 6 gender markers)
Random match probability
between 3.68E-17 and 4.23E-18 depending on population
On-target specificity
> 90 %
Coverage uniformity, within 2-fold of the median
98 %
Compatible platform
Illumina, AVITI
Number of samples per million read pairs
> 96 (at 30X coverage)
Workflow
The protocol consists of a single-tube reaction in which multiplex target amplification of polymorphic markers is combined with proprietary sample indexing to generate sequencing-ready libraries. All libraries are then equivolumetrically pooled, followed by clean-up, quantification, and sequencing. Software is available for analysis and reporting.
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